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KMID : 1149220190270030199
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Korean Journal of Oriental Medical Prescription
2019 Volume.27 No. 3 p.199 ~ p.211
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Study on the hepatoprotective effects of lemon balm and dandelion leaf extract combination in carbon tetrachloride-mediated liver injured mice
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Choi Beom-Rak
Cho Il-Je
Jung Su-Jin
Kim Jae-Kwang
Lee Dae-Geon
Ku Sae-Kwang
Park Ki-Moon
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Abstract
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Objectives : Present study investigated the hepatoprotective effects of various combinations of lemon balm and dandelion (LD) leaf extract on carbon tetrachloride (CCl4)-induced acute liver injury.
Methods : Mice were orally treated with 200 mg/kg of LD extracts [1:1, 1:2, 1:4, 1:6, 1:8, 2:1, 4:1, 6:1, or 8:1 (weight : weight)] for 7 days, and then intraperitoneally injected with CCl4 (0.5 mL/kg). Silymarin (100 mg/kg) was used as reference drug. Body weight gain, relative liver weight, serum biochemistry, histopathologic analyses, and hepatic antioxidant system were examined to elucidate the fittest combination ratio of LD extract.
Results : All varying combinations of LD extract significantly increased body weight gain and decreased relative liver weight by CCl4. In addition, LD extract reduced serum aspartate aminotransferase and alanine aminotransferase. Histopathological analyses indicated that LD extract significantly decreased histological activity index score, degenerated hepatocytes, and infiltrated inflammatory cells induced by CCl4. Moreover, LD extract reduced lipid peroxidation, and attenuated the reduction of hepatic glutathione, superoxide dismutase, and catalase by CCl4. Although there were no statistical differences in body weight gain between silymarin- and LD extract-treated groups, administration of 1:1, 2:1, and 4:1 combination of LD extract exhibited more favorable hepatoprotective effects than silymarin. Especially, 2:1 combination of LD extract showed the most potent hepatoprotective effects.
Conclusion : Of various combinations of LD extract tested, present results suggest that 2:1 combination of LD extract would be a promising herbal formulation to protect liver from oxidative stress.
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KEYWORD
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Acute liver injury, Carbon tetrachloride (CCl4), Dandelion leaf extract, Herbal combination, Lemon balm leaf extract.
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